Ten consecutive, unstained, unbaked FFPE tissue rolls cut at 10-micron thickness sections placed on positively charged slides are required. If the surface of the tissue section is above 0.5 cm2, 5 tissue rolls at 10-micron thickness are sufficient. One serial H&E is also required to assess specimen adequacy and to mark the tumor area for DNA extraction.

Submit a formalin-fixed, paraffin-embedded (FFPE) block containing tumor tissue. If a block is not available and examination of a representative H&E tissue section reveals that more than 50% of the cells are tumor cells, submit 10 FFPE tissue rolls cut at 10-micron thickness in a clean 1.5 ul tube. If examination of a representative H&E tissue section reveals that less than 50% of the cells are tumor cells, submit 10 FFPE unstained sections at 10-microns thickness on regular slides, unbaked and one H&E slide obtained as serial sections.

Submit a formalin-fixed, paraffin-embedded (FFPE) block containing tumor tissue (preferred specimen). If a block is not available submit 10 FFPE unstained sections at 10-micron thickness on regular slides, unbaked and one regular H&E obtained as serial sections; store at room temperature. Most insurance carriers require prior authorization for payment. Testing will not begin until insurance prior authorization is received, it is confirmed that prior authorization is not required, or the patient has agreed to pay out of pocket. A completed Michigan Medicine Request and Consent for Genetic Testing form is required and is available by calling 800-862-7284 or online: https://mlabs.umich.edu/media/188.

-Samples with less than 30% malignant cells or the tumor area is less than 1mm2 are not suitable for CGH. -Samples with total DNA yield less than 80ng and concentration less than 12ng/ µl, may be rejected due to the minimum DNA input requirements of CGH.